Bacterial 16s rRNA gene abundance based on quantitative PCR data from incubations with hydrocarbons

DNA-based stable-isotope probing with uniformly (13)C-labelled hydrocarbons followed by DNA sequencing was used to identify oil degrading bacteria present in surface and oil plume water associated with the Deepwater Horizon blowout. Further, qPCR specific to the groups identified as being involved in oil degradation via DNA-SIP was performed to show these groups respond to oil addition with increased abundance. This dataset includes 16S rRNA gene abundances acquired via qPCR. Data were generated through laboratory incubations of surface water collected near 28° 4.175N 88°22.335 W on May 5th 2010 and within plume water collected on May 31st 2010 at 28° 41.686 N 88° 26.081 W. The dataset listed under this UDI: R1.x132.135:0001 - is exclusive to Hydrocarbon-degrading bacterial DNA sequence and abundance.

本研究采用均匀13C标记烃类的DNA稳定同位素探针技术（DNA-stable-isotope probing，DNA-SIP）结合DNA测序，鉴定了与深水地平线（Deepwater Horizon）井喷事件相关的表层海水及油羽水中的石油降解细菌。此外，针对经DNA-SIP鉴定出的参与石油降解的细菌类群，开展了特异性定量PCR（quantitative PCR，qPCR）实验，结果显示此类群在添加烃类后丰度显著升高。本数据集包含通过qPCR获得的16S rRNA基因丰度数据。实验数据源自2010年5月5日采集于28°4.175′N、88°22.335′W附近的表层海水，以及2010年5月31日采集于28°41.686′N、88°26.081′W的油羽水的实验室培养实验。本唯一数据标识符（Unique Data Identifier，UDI）R1.x132.135:0001下收录的数据集，仅涵盖烃类降解细菌的DNA序列及丰度相关数据。