Expression analysis of wild type Fusarium verticillioides cultured in liquid media

Investigation of whole genome gene expression level changes over time of Fusarium verticillioides wild-type in liquid fumonisin inducing media (GYAM). Fusarium verticillioides produces a polyketide derived mycotoxin, fumonsin, over time in liquid media (Proctor et al, Co-expression of 15 contiguous genes delineates a fumonisin biosynthetic gene cluster in Gibberella moniliformis, Fungal Genetics and Biology, 38:237-249). A twelve chip study using total RNA recovered from twelve wild type cultures of Fusarium verticillioides. Each chip measures the expression level of 13,288 genes with thirteen 60-mer probe pairs per gene.

本研究针对轮枝镰孢（Fusarium verticillioides）野生型菌株在液体伏马菌素（fumonisin）诱导培养基（GYAM）中的全基因组基因表达水平随时间的动态变化展开分析。轮枝镰孢可在液体培养基中随时间合成聚酮衍生真菌毒素——伏马菌素（Proctor等，《15个连续基因的共表达划定赤霉（Gibberella moniliformis）伏马菌素生物合成基因簇》，《真菌遗传学与生物学（Fungal Genetics and Biology）》，第38卷，第237-249页）。本研究采用从12株轮枝镰孢野生型培养物中提取的总RNA，开展了12张基因表达芯片的检测实验；每张芯片可检测13288个基因的表达水平，每个基因对应13对60聚体探针。