methionine sulfoxide - Redox proteomics of protein-bound methionine oxidation

This project contains the COFRADIC data of two experimental set-ups (swapped labeling) in which methionine sulfoxides were identified in Jurkat cells stressed with hydrogen peroxide. We used a SILAC set-up in which cells were labeled with 12C5 methionine or with 13C5 methionine. One of the set-ups was treated with hydrogen peroxide, the other with H2O. Following lysis proteins were mixed in a 1/1 ratio. We then performed our methionine sulfoxide validation protocol onto the sample identifying methionine sulfoxides in the stressed Jurkat cells.

本数据集包含两种采用交换标记法（swapped labeling）的实验设置的COFRADIC数据，相关实验在经过氧化氢胁迫的Jurkat细胞中鉴定甲硫氨酸亚砜。我们采用了SILAC（Stable Isotope Labeling with Amino Acids in Cell Culture）实验体系，将细胞分别以12C5-甲硫氨酸（12C5 methionine）和13C5-甲硫氨酸（13C5 methionine）进行标记。其中一组实验经过氧化氢处理，另一组则以H₂O处理。细胞裂解后，将蛋白质按1:1的比例混合。随后我们针对该样本执行甲硫氨酸亚砜验证流程，最终在受胁迫的Jurkat细胞中成功鉴定出甲硫氨酸亚砜。