MOESM1 of Characterization of resistance to a potent d-peptide HIV entry inhibitor

Additional file 1. Mutations observed in PIE12-trimer resistant viral pools. Env gene position (pNL4-3 numbering), reference base, and all base calls for control and PIE12-trimer resistance viral populations with a mutation > 10% of the population (with percentage of the base calls for each position colored on a spectrum from low (light pink) to high (red)) are indicated. Additionally, the positions are annotated by their location in Env according to the following categories: known or putative glycosylation site (green), gp120 variable loops (gray), chemokine receptor (co-receptor) binding site (blue), rev-response element (dark gray), gp41 N-trimer (orange), gp41 C-peptide region (light blue), or the Rev coding region included within the env gene (yellow). Eighty-one positions were identified. Of these, 74 conform to our analysis criteria (found in the resistant pool(s) and > 10% different frequency than in the control pool).附加文件1. PIE12-三聚体抗性病毒库中观察到的突变。标注了Env基因的位置（采用pNL4-3编号体系）、参考碱基，以及对照病毒群体和PIE12-三聚体抗性病毒群体中突变占群体比例≥10%的所有碱基检出结果（每个位点的碱基检出百分比以光谱色标绘：低频率为浅粉色，高频率为红色）。此外，根据Env蛋白的位置将这些位点分为以下类别进行注释：已知或推定的糖基化位点（绿色）、gp120可变环（灰色）、趋化因子受体（共受体）结合位点（蓝色）、Rev应答元件（深灰色）、gp41 N-三聚体（橙色）、gp41 C肽区域（浅蓝色），以及env基因内包含的Rev编码区（黄色）。共鉴定出81个位点，其中74个符合本研究的分析标准（即在抗性病毒库中检出，且频率与对照库差异≥10%）。