eEF2K regulates pain through translational control of BDNF Smith et al.

Raw image files relating to Figure 2 eEF2K attenuates translation downstream of mGluR5, Figure 3 EF2K triggers the eIF2α phosphorylation through GCN2, and Figure 4 eEF2K/GCN2 signaling promotes translation of Bdnf. For all images, channel 1 represents PRPH immunofluorescence, and channel 2 represents the quantified target (PLA signal, p-eEF2, puromycin, or p-eIF2alpha). Details for each can be found in the figure legend.

本数据集包含与以下图件相关的原始图像文件：图2（真核延伸因子2激酶（eEF2K）抑制代谢型谷氨酸受体5（metabotropic glutamate receptor 5, mGluR5）下游的翻译过程）、图3（eEF2K通过通用调控非阻抑2蛋白（General Control Nonderepressible 2, GCN2）介导真核起始因子2α（eukaryotic initiation factor 2α, eIF2α）的磷酸化）以及图4（eEF2K/GCN2信号通路促进脑源性神经营养因子（Brain-derived neurotrophic factor, Bdnf）的翻译）。所有图像中，通道1为PRPH免疫荧光染色，通道2代表待定量靶标信号，包括原位 proximity连接测定（Proximity Ligation Assay, PLA）信号、磷酸化eEF2（phospho-eEF2, p-eEF2）、嘌呤霉素（puromycin）信号或磷酸化eIF2α（phospho-eIF2α, p-eIF2α）。各图像的详细信息请参见图注。