TMK4-mediated Phosphorylation of FIP37 Regulates Auxin-triggered N6-methyladenosine mRNA Modification of Auxin Biosynthetic Genes in Arabidopsis. TMK4-mediated Phosphorylation of FIP37 Regulates Auxin-triggered N6-methyladenosine mRNA Modification of Auxin Biosynthetic Genes in Arabidopsis

Using m6A-seq, RNA-seq, we show that auxin can induce transcriptome-wide m6A reprogramming. Overall design: This series includes samples for two experiments: m6A-seq (two biological replicates for mock and NAA treated 15-day-old wild-type seedlings plus input controls); RNA-seq (three biological replicates for mock and NAA treated 15-day-old wild-type.).

本研究通过m6A测序（m6A-seq）与RNA测序（RNA-seq）技术，证实生长素（auxin）可诱导全转录组水平的m6A重编程。整体实验设计：本数据集涵盖两类实验：m6A测序（m6A-seq）实验：包含mock处理组与NAA处理组的15日龄野生型幼苗样本，每组设置2个生物学重复，并增设输入对照（input controls）；RNA测序（RNA-seq）实验：针对15日龄野生型幼苗，mock处理组与NAA处理组各设置3个生物学重复。