Genome-wide mapping of accessible chromatin in hTert-HME1 cell line treated with control or BRCA2 siRNAs and grown with or without EGF (epithelial growth factor)

Mammary epithelial cell line hTert-HME1 was transfected with Control or BRCA2 siRNAs then passaged four times (12 days), then were grown in mammary epithelial growth medium with or without EGF (epithelial growth factor) for 2 passages (6 days). Overall design: Control siRNA transfection grown in media +EGF (3 replicates), control siRNA transfection grown in media -EGF (3 replicates), BRCA2 siRNA transfection grown in media +EGF (3 replicates), BRCA2 siRNA transfection grown in media -EGF (3 replicates)

将hTert-HME1人端粒酶永生化乳腺上皮细胞系分别以对照小干扰RNA（small interfering RNA，siRNA）或BRCA2靶向siRNA进行转染，随后传代培养4次（共计12天）；之后将细胞置于添加或不添加表皮生长因子（epithelial growth factor，EGF）的乳腺上皮细胞生长培养基中，继续传代培养2次（共计6天）。
实验整体设计如下：设置四组实验样本，分别为对照siRNA转染且培养液添加EGF组（3个生物学重复）、对照siRNA转染且培养液不添加EGF组（3个生物学重复）、BRCA2靶向siRNA转染且培养液添加EGF组（3个生物学重复）、BRCA2靶向siRNA转染且培养液不添加EGF组（3个生物学重复）