Human Hematopoietic Stem Cell Vulnerability to Ferroptosis [Ribosome profiling]

To investigate the translatome change due to loss of MYSM1 in CD34+CD45RA-CD90+ cells We performed ribosome profiling followed by NGS sequencing where only ribosome protected RNA was extracted to make the library, which mostly are in active translation comparative ribosome profiling assay of MYSM1 or AAVS1 edited CD34+CD45RA-CD90+ cells with three biological replicates of each group

为探究CD34+CD45RA-CD90+细胞中MYSM1缺失所引发的翻译组（translatome）变化，我们开展了核糖体谱分析（ribosome profiling）结合下一代测序（NGS）的实验：实验仅提取受核糖体保护的RNA以构建文库，此类RNA大多处于活跃翻译状态。本研究针对经MYSM1编辑或AAVS1编辑的CD34+CD45RA-CD90+细胞进行对照核糖体谱分析，每组均包含3次生物学重复。