Characterization of in vitro cultured alveolar macrophages (AM) heterogeneity following Wnt3a treatment [single-cell RNA-seq]

To understand how Wnt signaling affects AM phenotype in vitro, we treated AMs pooled from 5 WT mice with or without Wnt3a for overnight and performed single-cell RNA sequencing analysis in the cells. Dimensionality reduction analysis of combined control or Wnt3a-treated AMs revealed eight clusters based on their gene expression pattern, and control or Wnt3a-treated AMs were almost mutually exclusive as visualized in UMAP plot. Overall design: Examined with control or Wnt3a-treated AMs cultured in vitro.

为探究Wnt信号通路（Wnt signaling）在体外（in vitro）对肺泡巨噬细胞（alveolar macrophages, AMs）表型的影响，我们将5只野生型（wild type, WT）小鼠的AMs混合后，分别用Wnt3a处理或不做处理，于体外过夜培养，随后对这些细胞开展单细胞RNA测序（single-cell RNA sequencing）分析。
对对照组与Wnt3a处理组的AMs合并样本进行降维分析后，基于基因表达模式可将其划分为8个细胞簇；UMAP（Uniform Manifold Approximation and Projection）可视化结果显示，对照组与Wnt3a处理组的AMs几乎完全相互排斥。
实验整体设计：对体外培养的对照组与Wnt3a处理组AMs进行检测分析。