SIRT6 and SIRT1 contribute to circadian gene expression

Comparison of the hepatic circadian transcriptomes reveals that SIRT6 and SIRT1 separately control transcriptional specificity, and therefore, define distinctly partitioned classes of circadian genes. Livers from WT and SIRT6 KO mice, and livers from WT and SIRT1 KO mice, were harvested over the circadian cycle at ZT 0, 4, 8, 12, 16 and 20 for gene expression analysis.

对肝脏昼夜节律转录组的比较分析显示，沉默信息调节因子6（SIRT6）与沉默信息调节因子1（SIRT1）可分别调控转录特异性，由此划分出两类互不重叠的昼夜节律基因类别。本数据集分别采集野生型（Wild Type, WT）与SIRT6基因敲除（Knock Out, KO）小鼠、野生型（WT）与SIRT1基因敲除（KO）小鼠的肝脏组织，在昼夜节律周期内的授时因子时间（Zeitgeber Time, ZT）0、4、8、12、16、20六个时间点进行取样，用于基因表达分析。