MiRNAs sequence of macrophages derived exosoms in WT group and Roquin-1 san:san group.

We reported the function of Roquin-1 in the miRNA-sorting of macrophages derived exosomes. At first, we used the supernatant of 929 cells to culture the bone marrow derived macrophages (BMDM) from bone marrow cells of WT and Roquin-1 san:san mice. Then, we isolated the macrophages derived exosomes by ultracentrifugation. At last, we performed Next-generation sequencing to detect the differences of miRNA-sorting between WT and Roquin-1 macrophages derived exosomes. miRNA profiles of macrophages derived exosomes in WT and Roquin-1 san:san mice [contributor] Nanjing Medical University

本研究报道了Roquin-1在巨噬细胞源外泌体miRNA分选（miRNA-sorting）过程中的功能。首先，我们使用929细胞的上清液培养野生型（wild type, WT）及Roquin-1 san:san小鼠的骨髓来源巨噬细胞（bone marrow derived macrophages, BMDM）。随后，我们通过超速离心（ultracentrifugation）分离巨噬细胞源外泌体（exosomes）。最后，我们采用下一代测序（Next-generation sequencing）检测野生型与Roquin-1 san:san小鼠巨噬细胞源外泌体之间的miRNA分选差异。野生型及Roquin-1 san:san小鼠巨噬细胞源外泌体的miRNA表达谱 [贡献方] 南京医科大学