Genome-wide maps of histone modifications in WT, Dnmt3a-/- and Tet1-/- J1 ES cells.. Mus musculus

Using ChIP-seq to assess changes of histone modifications in response to Dnmt3a or Tet1 deficiency. Overall design: Examination of H3K4me3 and H3K27me3 in WT, Dnmt3a-/- and Tet1-/- ES cells.

本研究采用染色质免疫共沉淀测序（ChIP-seq）技术，评估DNA甲基转移酶3α（Dnmt3a）或Ten-eleven易位蛋白1（Tet1）缺失所导致的组蛋白修饰变化。实验设计概述：检测野生型（WT）、Dnmt3a基因敲除（Dnmt3a-/-）及Tet1基因敲除（Tet1-/-）胚胎干细胞（ES cells）中的组蛋白H3赖氨酸4三甲基化（H3K4me3）与组蛋白H3赖氨酸27三甲基化（H3K27me3）修饰水平。