CoBATCH for high-throughput single-cell epigenomic profiling [low input in situ ChIP]

we develop a robut low input cell and high throughput single cell ChIP method used for both native and fixed in vitro cultured cells and intact tissue Overall design: we fused Protein A to the N-terminal of Tn5 to generated Protein A-Tn5 fusion enzyme, the ProteinA-Tn5-complex is enriched through specific antibodies to genomic regions and Tn5 generates indexed chromatin fragments ready for the library preparation and sequencing; ROC - receiver operating characteristic.本研究开发了一种稳健型低起始量、高通量单细胞染色质免疫沉淀（Chromatin Immunoprecipitation, ChIP）方法，可应用于天然及固定的体外培养细胞与完整组织样本。整体实验设计：我们将蛋白A（Protein A）融合至Tn5转座酶的N端，制备得到蛋白A-Tn5融合酶；该复合物可通过特异性抗体富集至基因组区域，由Tn5生成带索引的染色质片段，用于后续文库制备与测序；ROC即受试者工作特征（Receiver Operating Characteristic）。