Effect of Cyclohexidmide treatment on early transcription of HSV-1 ?ICP22 and repaired virus

Study was performed to assess the requirement for HSV-1 IE protein, ICP22, to be expressed to regulate early HSV-1 transcription Overall design: PRO-Seq was performed on nuclei extracted at 1.5 hpi from HEp-2 cells infected with ?ICP22 HSV-1 mutant and its genetically restored repair. Infections occurred at an MOI of 5 in the presence or absence of the protein synthesis inhibitor cycloheximide (CHX)

本研究旨在评估单纯疱疹病毒1型（HSV-1）即刻早期蛋白ICP22的表达需求，以调控HSV-1的早期转录过程。整体实验设计如下：采用PRO-Seq技术，对感染ICP22缺失型HSV-1突变株及其基因修复株的HEp-2细胞，于感染后1.5小时（hours post infection, hpi）提取的细胞核进行转录分析。所有感染实验均以感染复数（MOI）为5的比例进行，并设置添加与不添加蛋白质合成抑制剂环己酰亚胺（cycloheximide, CHX）的实验组与对照组。