Results of qRT-PCR analysis.

= 7). Array p-values are based on a Bayesian two-tailed t-test, and TaqMan assays p-values are based on an unpaired t-test, corrected for multiple testing. Data were normalized to the average of the endogenous control genes indicated, based on qbasePLUS software's M scores. A, snoRNA142 (TaqMan assay ID: 001231); B, snoRNA234 (001234); C, U6 snRNA (001973). ID, Identification number; FC, fold change. P-values in italics, p<0.05. Confirmation of differential expression for selected miRNA with real-time PCR. Total RNA from cortex samples was used, and all 7 samples for each experimental group were included (number of datapoints per subgroup, n

(n=7)。阵列检测的P值基于贝叶斯双侧t检验(Bayesian two-tailed t-test)，TaqMan检测(TaqMan assay)的P值则基于经多重检验校正的非配对t检验。本数据以qbasePLUS软件的M评分为依据，对所标注的内参基因(endogenous control genes)的平均值进行归一化处理。A：核仁小RNA142(snoRNA142，TaqMan检测编号：001231)；B：核仁小RNA234(snoRNA234，编号：001234)；C：U6小核RNA(U6 snRNA，编号：001973)。ID为识别编号(Identification number)；FC为倍数变化(fold change)。斜体标注的P值表示p<0.05。采用实时荧光定量PCR(real-time PCR)对筛选出的微小RNA(miRNA)的差异表达进行验证。实验使用皮层样本的总RNA，每个实验组的全部7份样本均被纳入分析(每个亚组的数据点数量为n