The gene regulatory landscape of kidney organoid differentiation revealed by single nucleus multiomic analyses [multi-omics]

We performed single nucleus RNA sequencing (snRNA-seq) and assay for transposase-accessible chromatin with sequencing (snATAC-seq) to map the epigenetic and gene expression signatures of human kidney organoids during the organoid differentiation time course. Expression profiling by high throughput sequencing

本研究针对人类肾脏类器官的分化时间进程，采用单细胞核RNA测序（single nucleus RNA sequencing, snRNA-seq）与转座酶可及性染色质测序（assay for transposase-accessible chromatin with sequencing, snATAC-seq），绘制其表观遗传与基因表达特征图谱。本研究通过高通量测序开展基因表达谱分析。