Antibody Validation for Cyclical Immunofluorescence Microscopy of Human Kidneys (Part 1, Figures 31 and 33)

This dataset includes Cyclical Immunofluorescnce (CyCIF) images (15-20 channels) of frozen human kidney sections interogated with a panel of 12 validated antibodies designed to evaluate renal tubular cell segmentation (Figures 31 and 32), an a panel of 12 validated antibodies and a lectin designed to evaluate glomerular and glomerulus-associated structures in the normal human kidney (Figure 33). We have attached an excel file (Supplemental Table 2) that includes all of the experimental and (de-identified) patient metadata associated with these images, with information and comments about each of the channel images, the antibodies used, CyCIF cycles, and the cell types and extracellular matrix compartments identified with these combinations of antibodies. Because of the sixze of the images, we have divided this into two separate datasets (Antibody Validation for Cyclical Immunofluorescence Parts 1 and 2).

本数据集包含经12种经过验证的抗体组合标记检测的循环免疫荧光（Cyclical Immunofluorescence，CyCIF）图像（含15~20个通道），这些图像源自冰冻人肾脏切片，用于评估肾小管细胞分割效果（对应图31与图32）；此外还包含另一组标记组合，由12种经过验证的抗体与一种凝集素（lectin）组成，用于评估正常人体肾脏中的肾小球及肾小球相关结构（对应图33）。我们已附加一份Excel文件（补充表2），其中收录了与这些图像相关的全部实验数据及去标识化（de-identified）的患者元数据，涵盖各通道图像信息、所用抗体详情、CyCIF循环流程，以及通过这些抗体组合鉴定得到的细胞类型与细胞外基质区域的相关说明与注释。由于图像文件体量较大，我们将本数据集拆分为两个独立子数据集：《循环免疫荧光抗体验证集 第1部分》与《循环免疫荧光抗体验证集 第2部分》。