(CTA) expression after exposure to 5-aza-2'-deoxycytidine in HCT116 cells. Homo sapiens

The expression of CTAs is normally restricted to gametogenic tissue but is often reactivated in the tumorigenic setting. This tumorigenic reactivation is thought to be due to global demethylation events during tumorigenesis. To test this, we employed an affymetrix microarray in the hypermethylated colorectal carcinoma tumor-derived cell line, HCT116, following 5-aza-2'-deoxycytidine treatment. We used microarray analysis to uncover genes whose expression is modulated by DNA methylation in the colorectal carcinoma tumor derived cell line, HCT116. Overall design: HCT116 cells were exposed to 5-aza-2'-deoxycytidine for 72 hours. Total RNA was isolated and hybridized on to Affymetrix microarrays.

癌-睾丸抗原（Cancer/Testis Antigens, CTAs）的表达通常仅局限于生精组织，但在肿瘤发生环境中常被重新激活。这种肿瘤相关的重激活现象被认为由肿瘤发生过程中的全局去甲基化事件所介导。为验证这一假说，我们在高甲基化结直肠癌肿瘤来源细胞系HCT116中，经5-氮杂-2'-脱氧胞苷处理后，采用Affymetrix微阵列（Affymetrix microarray）开展实验。本研究借助微阵列分析，挖掘结直肠癌肿瘤来源细胞系HCT116中受DNA甲基化调控的表达基因。实验整体设计：将HCT116细胞置于5-氮杂-2'-脱氧胞苷环境中培养72小时，随后提取总RNA并与Affymetrix微阵列进行杂交。