Bone Marrow Derived Mesenchymal Stem Cells Incorporate Into the Prostate During Regrowth. Mus musculus

Transcriptional profiling of mouse prostate stromal cells knocked out for Tgfbr2 were compared to that of the Tgfbr2-flox control. Elevated chemokine expression in the prostate stroma of a castrate resistant mouse model, Tgfbr2-fspKO, prompted us to determine the role of bone marrow derived cell recruitment to the prostate during regrowth. Overall design: Tgfbr2-flox prostate epithelia vs. Tgfbr2-fspKO prostae epithelia. Biological replicates: 3 Tgfbr2-flox mouse prostates pooled, 3 Tgfbr2-fspKO mouse prostate replicates.

本研究对Tgfbr2（Transforming Growth Factor Beta Receptor II）敲除的小鼠前列腺基质细胞的转录组谱分析（Transcriptional profiling）结果，与Tgfbr2-flox对照样本进行了对比。在去势抵抗性小鼠模型Tgfbr2-fspKO的前列腺基质中，趋化因子（chemokine）表达水平显著升高，该发现促使我们探究骨髓源性细胞（bone marrow derived cell）在前列腺再生过程中向前列腺招募的作用。实验设计概述：将Tgfbr2-flox小鼠前列腺上皮与Tgfbr2-fspKO小鼠前列腺上皮进行对比。生物学重复设置：将3只Tgfbr2-flox小鼠的前列腺组织混合为一个样本，同时设置3份Tgfbr2-fspKO小鼠的前列腺组织作为生物学重复样本。