Landscape and age dynamics of immune cells in the Egyptian Rousette Bat

Bats harbor high-impact zoonotic viruses in absence of clinical disease, which has been recently associated with unique features of their immune system. They seem to restrict inflammation and possibly limit disease manifestation to a minimum. In-depth characterization of cellular immunity in bats is yet largely missing, and imprinting of age and development on immune cell compartments remains unexplored. We employed single-cell transcriptomics and established immunostaining panels to investigate the immune cell populations peripheral blood for juvenile and adult Egyptian Rousette bats (ERB). Total of 4 samples as 4 different 10x gene expression libraries prepared from ERB blood. Libraries A1 and A2 contain data about single-cell leukocytes extracted from peripheral blood of respectively three ERB adults and three ERB juveniles. Both blood collection and preparation of A1/A2 libraries were conducted on parallel on the same day. Library A5 contains data about single-cell leukocytes extracted from peripheral blood of one ERB adult and two ERB subadults. Library A6 contains data about single-cell leukocytes collected from the PBMC fraction of one ERB adult and two ERB subadults. Libraries A5 and A6 were prepared on the same day from the same 3 animals.

蝙蝠可携带可引发重大公共卫生影响的人畜共患病毒却不表现临床症状，这一现象近年被证实与其免疫系统的独特特征密切相关。该类动物能够有效抑制炎症反应，并可将疾病病理表现尽可能降至最低。目前学界对蝙蝠细胞免疫的深度特征解析仍存在大量空白，而年龄与发育进程对免疫细胞亚群的塑造作用仍有待探究。本研究借助单细胞转录组学技术，并构建免疫染色检测组合，以探究幼年与成年埃及Rousette蝙蝠（Egyptian Rousette Bat, ERB）外周血中的免疫细胞群。本数据集共包含4份样本，对应4个基于ERB血液构建的10x基因表达文库。A1与A2文库的数据来自分别从3只成年ERB蝙蝠与3只幼年ERB蝙蝠的外周血中分离的单细胞白细胞；上述两个文库的血液采集及文库构建流程均于同日并行开展。A5文库的数据来自从1只成年ERB蝙蝠与2只亚成年ERB蝙蝠的外周血中分离的单细胞白细胞。A6文库的数据来自从1只成年ERB蝙蝠与2只亚成年ERB蝙蝠的外周血单个核细胞（PBMC, Peripheral Blood Mononuclear Cell）组分中分离的单细胞白细胞。A5与A6文库均于同日使用同一批3只实验动物构建完成。