Gene expression changes by Glyceollin I treatment of breast cancer cells

We examined how glyceolin I, a phytoalexin from soybeands, affects gene expression in breast cancer cells. mRNA was collected at time points of 0, 6, 12, and 24 hours, and mRNA-seqs were performed. Overall design: Comparative gene expression profiling analysis of RNA-seq data at 0, 6, 12, and 24 hours with Glyceollin I treatment in MCF7, MCF7-LTED, and MDA-MB-468 cells.

本研究探讨了大豆来源的植物抗毒素Glyceollin I（大豆抗毒素I）对乳腺癌细胞基因表达的影响。研究人员于0、6、12及24小时四个时间点收集mRNA，并完成mRNA-seq测序实验。实验整体设计：针对MCF7、MCF7-LTED与MDA-MB-468三种细胞系，对经Glyceollin I处理后的样本，基于0、6、12、24小时四个时间点的RNA-seq数据开展比较基因表达谱分析。