Role of Tubular Cpt1a in Aging Murine Kidneys. Role of Tubular Cpt1a in Aging Murine Kidneys

To compare the transcriptional profile in aged kidneys lacking tubular Cpt1a with floxed littermate controls. Overall design: All mice were males and on a mixed background. Kidneys were either from mice that had Cpt1a deleted specifically in renal tubules (crossing Cpt1a floxed mice with Pax8-rTTA;tetO-Cre, which will be called Cpt1aCKO) or floxed controls. They had been treated for 3 weeks with doxycycline to activate Cre at age5-8 weeks.本研究旨在对比肾小管特异性缺失Cpt1a的衰老肾脏与同窝loxP侧翼修饰的（floxed）对照小鼠的肾脏转录组谱（transcriptional profile）。实验设计概述：所有实验小鼠均为雄性，且处于混合遗传背景下。实验采集的肾脏分别来自两类小鼠：一类为肾小管特异性缺失Cpt1a的小鼠（通过将Cpt1a floxed小鼠与Pax8-rTTA;tetO-Cre品系小鼠杂交构建，该基因工程小鼠品系命名为Cpt1aCKO），另一类为floxed对照小鼠。所有小鼠均在5~8周龄时以多西环素处理3周以激活Cre重组酶。