Transcriptome analysis of Saccharomyces cerevisiae petites. Transcriptome analysis of Saccharomyces cerevisiae petites

Two methods were used to induce petites from S, cerevisiae wild type strain MK288:.(1) MK288 was spread on YPD plate supplemented with 8 μg/ml fluconazole (2) MK288 was grown in YPD broth supplemented with 25 μg/ml ethidium bromide. Transcriptomes of the petites (TJ140 and TJ235) were compared to the wild type.

本研究采用两种方法从酿酒酵母（Saccharomyces cerevisiae）野生型菌株MK288中诱导得到小菌落突变体（petite）：(1) 将MK288涂布于添加8 μg/ml氟康唑的YPD平板上；(2) 将MK288在添加25 μg/ml溴化乙锭的YPD液体培养基中培养。随后，将两株小菌落突变体TJ140与TJ235的转录组与野生型菌株的转录组进行对比分析。