Table_5_Natural Variation of Lignocellulosic Components in Miscanthus Biomass in China.xlsx

Lignocellulose content is an important factor affecting the conversion efficiency of biomass energy plants. In this study, 179 Miscanthus accessions in China were used to determine the content of lignocellulose components in stems via acid hydrolysis and high-performance liquid chromatography. Results showed that the average lignocellulose content of wild Miscanthus germplasm resources was 80.27 ± 6.51%, and the average content of cellulose, hemicellulose, lignin, extracts, and total ash was 38.38 ± 3.52, 24.23 ± 4.21, 17.66 ± 1.56, 14.50 ± 5.60, and 2.53 ± 0.59%, respectively. The average lignocellulose content of M. sinensis, M. floridulus, M. nudipes, M. sacchariflorus, M. lutarioriparius, and the hybrids was 77.94 ± 6.06, 75.16 ± 4.98, 75.68 ± 3.02, 83.71 ± 4.78, 81.50 ± 5.23, and 74.72 ± 7.13%, respectively. In all the tested materials, the highest cellulose content was 48.52%, and the lowest was 29.79%. Hemicellulose had the maximum content of 34.23% and a minimum content of 15.71%. The highest lignin content was 23.75%, and the lowest was 13.01%. The lignocellulosic components of different ploidy materials were compared. The content of lignocellulosic components of diploid M. sacchariflorus was higher than that of tetraploid M. sacchariflorus, and the content of lignocellulosic components of diploid M. lutarioriparius was lower than that of tetraploid M. lutarioriparius. Analysis of the relationship between the changes in lignocellulosic components and geographical locations of Miscanthus showed that the holocellulose and hemicellulose content was significantly positive correlated with the latitude of the original growth location. Results indicated that the lignocellulosic components of Miscanthus resources in China are rich in genetic diversity.

木质纤维素（lignocellulose）含量是影响生物质能源植物转化效率的关键因素。本研究以中国境内的179份芒草种质为材料，采用酸水解法结合高效液相色谱法测定其茎秆中的木质纤维素组分含量。结果显示，野生芒草种质资源的木质纤维素平均含量为80.27±6.51%；其中纤维素、半纤维素、木质素、提取物及总灰分的平均含量分别为38.38±3.52%、24.23±4.21%、17.66±1.56%、14.50±5.60%及2.53±0.59%。中国芒（M. sinensis）、五节芒（M. floridulus）、裸穗芒（M. nudipes）、荻（M. sacchariflorus）、南荻（M. lutarioriparius）及其杂交种的木质纤维素平均含量分别为77.94±6.06%、75.16±4.98%、75.68±3.02%、83.71±4.78%、81.50±5.23%及74.72±7.13%。供试材料中，纤维素含量最高达48.52%，最低为29.79%；半纤维素含量最大值为34.23%，最小值为15.71%；木质素含量最高为23.75%，最低为13.01%。本研究对不同倍性材料的木质纤维素组分含量进行了比较，发现二倍体荻的木质纤维素组分含量高于四倍体荻，而二倍体南荻的木质纤维素组分含量则低于四倍体南荻。通过分析木质纤维素组分变化与芒草地理分布的相关性，结果显示全纤维素（holocellulose）与半纤维素含量与材料原生境纬度呈显著正相关。研究结果表明，中国境内的芒草资源木质纤维素组分具有丰富的遗传多样性。