Proteomic analysis of Pacific oyster (Crassostrea gigas) mucus during Vibrio infection

This study aimed to identify the protein composition of mucus in Crassostrea gigas (Pacific oyster) and analyze the proteomic changes during Vibrio ZJ-51 infection. Mucus samples were collected from control (C1–C3) and infected oysters (T1–T3). Using LC-MS/MS, over 4000 proteins were identified from oyster mucus. Differential expression analysis revealed significant proteomic alterations in response to Vibrio infection, providing insights into the host-pathogen interaction and potential defense mechanisms. This study contributes to the understanding of oyster mucus composition and its response to bacterial challenge.

本研究旨在鉴定长牡蛎（Crassostrea gigas，Pacific oyster）粘液的蛋白质组成，并分析其在弧菌ZJ-51（Vibrio ZJ-51）感染过程中的蛋白质组变化。研究采集了对照组（C1–C3）与感染组（T1–T3）的长牡蛎粘液样本，采用液相色谱-串联质谱（LC-MS/MS）从长牡蛎粘液中鉴定出超过4000种蛋白质。差异表达分析结果显示，弧菌ZJ-51感染后宿主蛋白质组发生显著改变，为宿主-病原体相互作用及潜在防御机制提供了新的见解。本研究有助于加深对长牡蛎粘液组成及其应对细菌侵染的响应的理解。