Genome wide mapping of histone mark and transcription factor binding in the context of replicative senescence in IMR90 cells.

Analysis of histone and transcription factor epigenomic landscape of IMR90s from young to replicative senescent IMR90s were collected at young-quiescent and senescent stages, at which point chromatin was harvested and prepared for ChIP-seq against a variety of histone marks and transcription factors. ChIP-seqs of histone marks H3K27ac and H3K4me2 were performed in duplicate, and, in all experiments, senescent samples were compared against young samples for each factor.

本数据集针对IMR90细胞从年轻状态至复制性衰老状态的组蛋白与转录因子表观基因组景观开展分析：研究人员采集了处于年轻静息态与衰老态的IMR90细胞样本，于该阶段提取其染色质，针对多种组蛋白修饰标记与转录因子制备样本并开展染色质免疫共沉淀测序（ChIP-seq）实验。其中组蛋白修饰标记H3K27ac与H3K4me2的ChIP-seq实验设置了生物学重复；所有实验均针对每个靶标因子，以年轻样本为对照，与对应衰老样本进行比较分析。