Temporal single cell transcriptome atlas of zebrafish anterior segment development reveals high degree of conservation between the trabecular meshwork and the annular ligament. Temporal single cell transcriptome atlas of zebrafish anterior segment development reveals high degree of conservation between the trabecular meshwork and the annular ligament

Anterior segment dysgenesis (ASD), resulting in vision impairment, stems from maldevelopment of anterior segment (AS) tissues. Incidence of ASD has been linked to malfunction of periocular mesenchyme cells (POM). POM cells specify into anterior segment mesenchyme (ASM) cells which colonize and produce AS tissues. In this study we uncover ASM developmental trajectories associated with formation of the AS Overall design: Using a transgenic line of zebrafish that fluorescently labels the ASM throughout development, Tg[foxc1b:GFP], we isolated GFP+ ASM cells at several developmental timepoints (48-144hpf) and performed single cell RNA sequencing

眼前段发育异常（Anterior segment dysgenesis, ASD）可引发视力受损，其根源为眼前段（anterior segment, AS）组织发育异常。已有研究证实，ASD的发病率与眼周间充质细胞（periocular mesenchyme cells, POM）功能紊乱密切相关。POM细胞可分化为眼前段间充质（anterior segment mesenchyme, ASM）细胞，此类细胞定植并形成眼前段组织。本研究揭示了与眼前段形成相关的ASM发育轨迹。实验总体设计：利用可在整个发育过程中对ASM进行荧光标记的转基因斑马鱼品系Tg[foxc1b:GFP]，在多个发育时间点（48~144hpf）分离GFP阳性的ASM细胞，并开展单细胞RNA测序。