Used the hepatopancreas from live Macrobrachium rosenbergii Transcriptome. Macrobrachium rosenbergii

De novo transcriptome sequencing generated 66,953 isogenes. Using BLASTX to search the NR, STRING, and KEGG databases; 21,224 unigenes were annotated, 9,552 matched unigenes with the GO classification; 5,782 matched unigenes in 25 categories of GOG and 20,859 unigenes were consequently assigned to 312 KEGG pathways. Between the A and B groups 598 DEGs were identified; between the A1 and A2 groups 6,860 DEGs were identified and between the B1 and B2 groups 5,229 DEGs were identified. After enrichment, A and B groups identified 38 DEGs in two main categories based on functional groups: biological process and molecular function, and KEGG pathway defined 61 genes had a KO ID and could be categorized into 41 pathways. A1 and A2 groups identified 22,086 DEGs in three main categories based on functional groups: biological process, cellular_component and molecular function and KEGG pathway defined 2,459 genes had a KO ID and could be categorized into 251 pathways. B1 and B2 groups identified 5,940 DEGs in three main categories based on functional groups: biological process, cellular_component and molecular function, and KEGG pathway defined 1,543 genes had a KO ID and could be categorized into 240 pathways. We investigated 7 DEGs in GO: 0009987 which related to growth of GFP in 4 groups

本研究通过从头转录组测序（de novo transcriptome sequencing）共获得66953条单基因（unigene，原文为isogenes，疑似笔误）。采用BLASTX比对NR、STRING及KEGG（京都基因与基因组百科全书）数据库进行注释，最终得到21224条被注释的单基因；其中9552条单基因可归入GO（基因本体）分类体系，5782条单基因被划分为25个GOG功能类别，另有20859条单基因被注释到312条KEGG通路中。组间差异表达基因（DEGs，differentially expressed genes）鉴定结果如下：A组与B组间共鉴定出598个，A1组与A2组间共鉴定出6860个，B1组与B2组间共鉴定出5229个。富集分析后，针对A组与B组的比较结果：基于功能类群可划分为2个主要类别（生物过程与分子功能），共包含38个差异表达基因；经KEGG通路注释，共有61个基因获得KO编号，可归类至41条通路中。针对A1组与A2组的比较结果：基于功能类群可划分为3个主要类别（生物过程、细胞组分及分子功能），共包含22086个差异表达基因；经KEGG通路注释，共有2459个基因获得KO编号，可归类至251条通路中。针对B1组与B2组的比较结果：基于功能类群可划分为3个主要类别（生物过程、细胞组分及分子功能），共包含5940个差异表达基因；经KEGG通路注释，共有1543个基因获得KO编号，可归类至240条通路中。本研究针对4个组中与GFP（绿色荧光蛋白，Green Fluorescent Protein）生长相关的GO:0009987条目下的7个差异表达基因开展了分析。