“Identifying the SLC25A46 interactome in transgenic mice: cerebellum”

SLC25A46 is an outer mitochondrial protein, member of the mitochondrial carrier family (SLC25), whereas mutations result in mitochondrial structure abnormalities leading to a wide spectrum of neurological disorders. SLC25A46 is known to interact with members of the MICOS (Mitochondrial Contact Site) complex involved in mitochondrial cristae organization, and components of the endoplasmic reticulum membrane. In the present work, our aim was to identify the interactome of SLC25A46 in cerebrum, cerebellum, heart and thymus from TgSLC25A46-FLAG transgenic mice through immunoprecipitation with anti-FLAG beads and proteomic analysis with LC-MS/MS.

SLC25A46是一种线粒体外膜蛋白，隶属于线粒体载体家族（SLC25），该蛋白的突变可引发线粒体结构异常，进而导致广谱神经系统疾病。已知SLC25A46可与参与线粒体嵴组织的MICOS（线粒体接触位点复合物，Mitochondrial Contact Site）复合体成员以及内质网膜组分发生相互作用。本研究旨在通过抗FLAG磁珠免疫沉淀结合液相色谱-串联质谱（LC-MS/MS）蛋白质组学分析，鉴定TgSLC25A46-FLAG转基因小鼠大脑、小脑、心脏及胸腺中SLC25A46的蛋白质互作组。