16S_repset.fa.zip

Representative 16S rRNA sequences. The V3-V4 hypervariable region of the bacterial 16SrRNA gene was amplified with 341F/805R primers. For detailed sequencing methods, see Delgado-Baquerizo et al 2018. Data were processed using the USEARCH10 pipeline. Unique sequences were identified using usearch10 -fastx_uniques, and clustered at 97% identity to “Operational Taxonomic Units” (OTUs) with usearch10.代表性16S核糖体RNA（16S rRNA）序列。针对细菌16S rRNA基因的V3-V4高变区，采用341F/805R引物进行扩增。具体测序方法详见Delgado-Baquerizo等人2018年的研究。测序数据采用USEARCH10分析流程进行处理。使用usearch10的-fastx_uniques参数识别唯一序列，并以97%的序列相似度将其聚类为操作分类单元（Operational Taxonomic Units，OTUs）。