Histone H3 lysine 9 dimethylation of miRNA genes in HMEC and HMF

Normal cell type specific histone H3 lysine 9 dimethylation of miRNA genes. HMEC and HMF represent two distinct differentiated cell type present in mammary gland each with a distinct phenotype, a distinct epigenotype as well as distinct miRNA expression pattern. The aim of the study was to determine how epigenetic modifications including histone H3 lysine 9 dimethylation affect miRNA expression. Two cell types HMEC vs. HMF. Biological replicates: 3 pairs of HMEC-HMF of 3 distinct genotypes. Immunoprecipitation using anti-histone H3 dimethylated at lysine 9 (CS200587, Millipore).

本数据集聚焦miRNA基因的正常细胞类型特异性组蛋白H3赖氨酸9二甲基化（histone H3 lysine 9 dimethylation）特征。HMEC与HMF为乳腺组织中存在的两种不同分化细胞类型，二者分别具有独特的表型、表观遗传特征以及miRNA表达谱。本研究旨在阐明包括组蛋白H3赖氨酸9二甲基化在内的表观遗传修饰对miRNA表达的调控机制。实验共包含两种细胞类型：HMEC与HMF。生物学重复设置为3组HMEC-HMF细胞对，对应3种不同基因型。实验采用靶向赖氨酸9二甲基化组蛋白H3的抗体（货号CS200587，密理博（Millipore）公司）完成免疫沉淀实验。