CD133+ and CD133- expression profiles of benign and HRPC cells. Homo sapiens

Epithelial cell cultures derived from benign and HRPC tissue biopsies were expanded in culture for 2-3 weeks. CD133+ and CD133- cells were isolated using the miltenyi magnetic bead system after adherence to collagen. CD133+ and CD133- RNA was isolated and amplified using the RiboAmp HS kit and expression profiling performed using the CRUK WGA gene set. Keywords: Tumour vs normal comparison Overall design: 28 samples - All samples compared to universal reference RNA

源自良性及激素难治性前列腺癌（HRPC）组织活检标本的上皮细胞培养物，于体外扩增培养2~3周。待细胞贴附于胶原包被底物后，采用美天旎（Miltenyi）磁珠分选系统分离CD133阳性与CD133阴性细胞。分别提取并扩增CD133阳性与CD133阴性细胞的RNA，使用RiboAmp HS试剂盒完成扩增操作，并通过英国癌症研究中心（CRUK）全基因组扩增（WGA）基因集开展表达谱分析。关键词：肿瘤与正常组织对照研究 实验设计概况：共纳入28份样本，所有样本均与通用参考RNA进行比对分析。