In vitro development and acclimatization of Cyrtopodium aliciae L. Linden & Rolfe, an endemic species of the Chapada Diamantina

ABSTRACT: Orchids are valued as ornamental plants, bioindicators, and medicinal plants, which implies that some species may be over-collected. Some inhabit very fragile environments and are under threat by the misuse of habitats and anthropogenic impacts. The search for beautiful plants and flowers has increased the number of facilities for micropropagation either by seeding or by cloning plants using in vitro techniques. However, not all species have appropriate media for growth and development that would help in conservation efforts. Cyrtopodium aliciae is an endemic species of rupestrian grassland in Brazil t. It has appeal as an ornamental plant or for use in hybridisation programs dueo its small size and white brownish-purple dotted flowers. This study compared three different media, namely ½ concentration Murashige and Skoog (MS), Vacin and Wendt, and Knudson C, during plant growth and their effect on the acclimatization of Cyrtopodium aliciae. The number and length of shoots and roots, increase in mass, and survival in vitro and ex vitro were analyzed. The experiment was conducted as completely random with a factorial arrangement of treatments (3 × 3) with 10 repetitions per treatment containing 10 plants for the in vitro experiment and 3 repetitions of 10 plants for the ex vitro experiment. Cyrtopodium aliciae performed better in the ½ concentration MS medium with a higher increase in mass, plant development, and survival under both in vitro and ex vitro conditions.摘要：兰花兼具观赏植物、生物指示植物与药用植物的多重价值，这使得部分物种面临过度采集的风险。部分兰花栖息于极为脆弱的生境中，正遭受生境滥用与人为活动的威胁。市场对美观植物与花卉的需求持续增长，推动了通过播种或离体克隆技术开展植物微繁殖的设施数量不断增加。然而，并非所有物种都拥有适配其生长发育的培养基，以助力物种保护工作。 Cyrtopodium aliciae是巴西岩生草原的特有物种，其体型小巧、花朵带有白棕紫色斑点，具备观赏价值或可应用于杂交育种项目。本研究对比了三种不同培养基——1/2浓度Murashige and Skoog（MS）培养基、Vacin and Wendt培养基以及Knudson C培养基——在植株生长阶段的表现，及其对Cyrtopodium aliciae移栽驯化的影响。研究分析了芽与根的数量及长度、生物量增量，以及离体培养和移栽驯化阶段的存活率。 实验采用完全随机因子设计，处理组合为3×3因子设计：离体培养实验中每个处理设置10次重复，每次重复包含10株植株；移栽驯化实验中每个处理设置3次重复，每次重复包含10株植株。结果显示，Cyrtopodium aliciae在1/2浓度MS培养基中表现更优，其生物量增量、植株生长状况以及离体培养和移栽驯化阶段的存活率均更高。