Environmental DNA metabarcoding analysis of vertebrates in Ishigaki and Okinawa Islands, Okinawa Prefecture, Japan. riverine metagenome

DNA metabarcoding sequencing analysis of river water samples based on vertebrate mitochondrial 12S rRNA partial gene amplified by MiFish primers. Thirty-four locations of the Ishigaki and Okinawa Islands were analyzed as the known or potential Leptospira-endemic area.

本研究针对河水样本开展基于MiFish引物（MiFish primers）扩增脊椎动物线粒体12S核糖体RNA（mitochondrial 12S rRNA）部分基因的DNA宏条形码（DNA metabarcoding）测序分析，研究区域涵盖石垣岛与冲绳岛的34个采样点位，上述区域均为已知或潜在的钩端螺旋体病流行区（Leptospira-endemic area）。