Effects of 100 µM NS 2028 on fluorescence intensity of FlAsH labelled haem- free TC4-Y135A/R139A sGC (A) and ReAsH labelled TC4-WT sGC (B) after 90 min incubation.

cGMP reporter cells were cotransfected with WT α1 sGC and the respective β1 sGC and labelled with FlAsH or ReAsH. Fluorescence intensity of single cells was monitored in a time series of 120 laser scans and is expressed as % of start value which was obtained before application of the test compounds. Data are means ± S.E.M. from 3–5 single cells assayed on different days.将环磷酸鸟苷（cyclic guanosine monophosphate, cGMP）报告细胞与野生型（Wild Type, WT）α1亚基可溶性鸟苷酸环化酶（soluble guanylyl cyclase, sGC）及对应β1亚基可溶性鸟苷酸环化酶共转染，随后采用FlAsH或ReAsH完成标记。对单个细胞的荧光强度开展120次激光扫描的时序监测，检测结果以受试化合物施加前获取的初始值的百分比形式呈现。实验数据为不同实验天数内检测的3~5个单个细胞的均值±标准误（Standard Error of the Mean, S.E.M.）。