Gene expression data from myocardial infarction porcine samples. Sus scrofa

The use of cDNA microarrays has made it possible to analyze expression of thousands of genes simultaneously. We employed microarray gene expression profiling of porcine cDNA to compare myocardial gene expression in infarct core and remote myocardium at 1 week (n=3), 4 weeks (n=3), and 6 weeks (n=3) after surgically induced myocardial infarction (MI) and in sham-operated controls (n=3). More than 8,000 cDNA sequences were identified in myocardium that showed differential expression in response to MI. Different temporal and spatial patterns of gene expression were recognized in the infarct core tissue within this large set of data. Microarray gene profiling revealed candidate genes, some of them described for the first time, which elucidate changes in biological processes at different stages after MI. Overall design: We evaluated temporal gene expression in a porcine model of myocardial infarction by microarray technology. Nine female pigs (Sus scrofa crossbreed Landrace x Large White) weighing 30-40 kg were subjected to experimental MI by a double-ligation of the first marginal branch of the circumflex artery. Animals were randomly sacrificed at 1 week (n=3), 4 weeks (n=3), or 6 weeks (n=3) after MI. Three paired myocardial samples from the infarct core and remote myocardium were analyzed at each time point. Myocardial samples from sham-operated animals (open-chest cardiac exposure without coronary artery ligation; n=3) were included as physiological controls.

互补DNA（complementary DNA，cDNA）微阵列技术的应用，使得同时分析数千个基因的表达水平成为可能。本研究采用猪源cDNA微阵列基因表达谱分析技术，对比手术诱导心肌梗死（myocardial infarction，MI）后1周（n=3）、4周（n=3）及6周（n=3）时的梗死核心区与远端心肌的基因表达水平，并设置假手术对照组（n=3）作为参照。
本研究在心肌组织中鉴定出超过8000条cDNA序列，其表达水平在心肌梗死应答过程中呈现显著差异。基于该大规模数据集，我们在梗死核心区组织中识别到不同的基因表达时空模式。微阵列基因谱分析筛选出一批候选基因，其中部分为首次报道，这些基因阐明了心肌梗死术后不同阶段的生物学过程变化。
实验整体设计：本研究借助微阵列技术，对心肌梗死猪模型中的基因表达时序特征进行评估。选取9只体重30~40 kg的雌性长白×大白杂交猪（Sus scrofa crossbreed Landrace x Large White），通过双重结扎冠状动脉回旋支的第一边缘支构建实验性心肌梗死模型。分别于心肌梗死后1周（n=3）、4周（n=3）及6周（n=3）随机处死实验动物；每个时间点均采集3对来自梗死核心区与远端心肌的组织样本进行分析。同时纳入3只假手术动物（仅开胸暴露心脏，不进行冠状动脉结扎）的心肌组织样本作为生理对照。