The small transcriptome of Escherichia coli in exponential and stationary phase

We have deep sequenced the small transcriptome of Escherichia coli growing in LB and in MOPS, in exponential and stationary phase, and analyzed the resulting reads by a novel pipeline STARPA (Stable RNA Processing Product Analyzer). Our analysis reveals over 14,000 small transcripts enriched during both growth stages. RNA samples were collected from total RNA pools or from crude ribosome pools and then size selected by electrophoresis to limit the products to 20-300nt. Overall design: Differential expression analysis demonstrates the presence of enriched small transcripts in stationary phase, likely mediating regulatory functions.

本研究对在LB培养基与MOPS培养基中、分别处于指数生长期与稳定生长期的大肠杆菌（Escherichia coli）小转录组进行了深度测序，并通过全新分析流程STARPA（Stable RNA Processing Product Analyzer，稳定RNA加工产物分析器）对所得测序读段（reads）开展分析。分析结果显示，两个生长阶段均富集了超过14000个小转录本。
RNA样本采集自总RNA混合池或粗制核糖体混合池，随后通过电泳进行片段大小筛选，将目标产物限定在20至300个核苷酸（nt）范围内。
整体设计：差异表达分析表明，稳定生长期存在富集的小转录本，其可能介导调控功能。