Trans-maternal exposure to Helicobacter pylori induces stable and highly suppressive regulatory T-cells and protects against allergic airway inflammation. Trans-maternal exposure to Helicobacter pylori induces stable and highly suppressive regulatory T-cells and protects against allergic airway inflammation

Purpose: The gastric microbe Helicobacter pylori represents an ancestral constituent of the human microbiota that causes gastric disorders on the one hand, and is inversely associated with allergies and chronic inflammatory conditions on the other. This study aims to investigate the consequences of trans-maternal exposure to H. pylori extract in utero and during lactation on the regulatory T-cell transcriptome profile. Experiment type: Expression profiling by high throughput sequencing Overall design: Transcriptome profling (RNA-seq) of lung regulatory T-cells in mice after perinatal PBS and H. pylori extract exposure. One factorial design with 2 levels (with and without H. pylori exposure) including 2-3 biological replicates per experimental group. A biological replicate represents pools from 3-4 animals.

研究目的：胃微生物幽门螺杆菌（Helicobacter pylori）是人类微生物群系的祖先组成部分，一方面可引发胃部疾病，另一方面则与过敏症及慢性炎症性疾病呈负相关关联。本研究旨在探究宫内及哺乳阶段经母体暴露于幽门螺杆菌提取物，对调节性T细胞转录组特征的影响。
实验类型：基于高通量测序的表达谱分析
实验设计：对围产期暴露于磷酸盐缓冲液（PBS）与幽门螺杆菌提取物的小鼠肺部调节性T细胞开展转录组测序（RNA-seq）分析。本实验采用单因素两水平析因设计（幽门螺杆菌暴露组与未暴露组），每组设置2-3个生物学重复；每个生物学重复由3-4只动物的样本混合制备而成。