pcr Sequences

The repair of maxillofacial bone defects has long been a pivotal and challenging frontier in oral medicine research. This study aims to clarify the regulatory role and mechanism of miR-224-5p in the osteogenic differentiation of human dental pulp stem cells (hDPSCs), thereby laying a theoretical foundation for subsequent jaw defect repair.In this study, plasmid transfection was used to regulate the expression of miR-224-5p in hDPSCs, which were then treated with osteogenic induction medium to induce osteogenic differentiation. To determinethe mRNA expression of runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), and osteocalcin (OCN), qRT‒PCR was performed. A dual-luciferase assay was conducted to verify the targeting relationship between PTEN and miR-224-5p, and the regulatory role of the PI3K/AKT pathway was confirmed through rescue experiments.

颌面部骨缺损修复长期以来一直是口腔医学研究领域兼具关键意义与挑战性的前沿方向。本研究旨在阐明miR-224-5p在人牙髓干细胞（human dental pulp stem cells, hDPSCs）成骨分化中的调控作用及其机制，从而为后续颌骨缺损修复奠定理论基础。
本研究采用质粒转染技术调控hDPSCs中miR-224-5p的表达，随后使用成骨诱导培养基处理细胞以诱导其成骨分化。为检测runt相关转录因子2（runt-related transcription factor 2, RUNX2）、碱性磷酸酶（alkaline phosphatase, ALP）及骨钙蛋白（osteocalcin, OCN）的mRNA表达水平，本研究采用qRT-PCR技术。
本研究通过双荧光素酶报告基因实验验证PTEN与miR-224-5p的靶向调控关系，并通过拯救实验证实PI3K/AKT信号通路的调控作用。