scGEX/scTCR-Seq of CD44+ cytotoxic T cells in LCMV-infected Wildtype and Il1rl1-ExAB-/- mice

To study the effect of IL-33 sensing on CD8 T cell differentiation during acute infection with lymphocytic choriomeningitis virus (LCMV), activated CD44+ CTLs were flowcytometrically sorted from infected C57BL/6 wildtype and Il1rl1 gene-targeted mice (Il1rl1-ExAB-/-) and subjected to combined single-cell gene expression and single cell TCR-Seq analysis. Comparative gene expression profiling analysis of the effects of IL-33 on CTLs, Th1 and Th2 cells using RNA-Seq and single-cell RNA-Seq of WT- and Il1rl1-ExAB-KO CTLs after an acute LCMV infection.

为研究淋巴细胞脉络丛脑膜炎病毒（LCMV）急性感染过程中IL-33感知对CD8阳性T细胞分化的影响，本研究从感染后的C57BL/6野生型小鼠及Il1rl1基因靶向敲除小鼠（Il1rl1-ExAB-/-）中经流式细胞术分选出活化的CD44阳性细胞毒性T淋巴细胞（CTLs），并对其开展联合单细胞基因表达与单细胞TCR测序（single-cell TCR-Seq）分析。此外，本研究通过对急性LCMV感染后野生型与Il1rl1-ExAB敲除型CTLs的RNA测序（RNA-Seq）及单细胞RNA测序（single-cell RNA-Seq），开展针对IL-33对CTLs、Th1及Th2细胞作用的比较基因表达谱分析。