Circulating miRNA signature in serum of pateints with primary sclerosing cholangitis (PSC), cholangiocarcinoma (CC) and healthy controls.

RNA was isolated from serum of pateints with PAS, CC and healthy controls. Global microRNA profiling was done using miRNome microRNA Profiler QuantiMir Human PCR Array (#RA660A-1, version 15; BioCat, Heidelberg, Germany) miRNA arrays were performed on pooled RNA samples from six patients for PSC and CC each and a comparison was performed to healthy control patients. MiRNAs with Ct value above 32 were discarded.从患有PAS、CC的患者及健康对照者的血清中分离核糖核酸（RNA）。采用miRNome microRNA Profiler QuantiMir人类PCR芯片（货号#RA660A-1，版本15；德国海德堡BioCat公司）开展全局微小RNA（microRNA，简称miRNA）表达谱分析。分别针对PSC和CC各6例患者的混合RNA样本进行miRNA芯片检测，并与健康对照患者进行对照比较。Ct值高于32的miRNA将被剔除。