Estradiol Induced Transcriptional Profile of EAAE DNA Binding Deficient ERa Uterus. Mus musculus

To evaluate the ability of a DNA binding deficient ERa to mediate transcriptional responses in the mouse uterus, ovariectomized mice were injected with 100 ul of saline or 250 ng of estradiol (E2) in 100 ul saline, uterine tissue was collected 2 hours filllowing the injection, and RNA was isolated Overall design: 3 mice each, WT saline, WT E2 2h, EAAE saline, EAAE 2h E2

为评估DNA结合缺陷型雌激素受体α（ERα）介导小鼠子宫内转录应答的能力，本研究对去卵巢小鼠分别注射100微升生理盐水，或100微升含250纳克雌二醇（E2）的生理盐水；于注射后2小时收集子宫组织并提取RNA。实验整体设计：每组各3只小鼠，具体分组为野生型（Wild Type，简称WT）生理盐水组、野生型E2处理2小时组、EAAE生理盐水组、EAAE E2处理2小时组。