Grapeseed Meal Used as Natural Antioxidant in High Fatty Acid Diets for Hubbard Broilers

ABSTRACT The 7-week feeding trial evaluated the effect of 2% grapeseed meal used as natural antioxidant in the diets for slow-growing Hubbard broiler chicks, aged 14 days. The chicks were weighed individually and assigned to two groups (C and E), with 40 chicks per group, housed under conditions of temperature, humidity and light regimen according to the hybrid management guide. The basal ingredients of the conventional diet were corn, wheat, gluten, soybean meal and 2% flaxseed meal, which is rich in polyunsaturated fatty acids. Compared to the control formulation, the experimental formulation included 2% grapeseed meal, both in the growing and finishing stages. The broilers had free access to feed and water. In the end of the feeding trial, blood samples were collected for serum biochemical determinations and six broilers per group were slaughtered and samples of breast and thigh meat were collected. The meat samples were assayed for the basic chemical composition, fatty acids profile and cholesterol content using standardized methods. The content of total polyunsaturated fatty acids (PUFAs) was significantly higher (p摘要 本试验为为期7周的饲养试验，旨在评估14日龄慢生长型哈伯德（Hubbard）肉鸡雏鸡日粮中添加2%葡萄籽粕作为天然抗氧化剂的饲喂效果。试验肉鸡均经个体称重后被随机分为两组（C组与E组），每组各40只，饲养环境的温度、湿度与光照制度均参照杂交肉鸡饲养管理指南设置。基础对照组日粮的成分为玉米、小麦、谷蛋白、豆粕以及2%亚麻籽粕，该日粮富含多不饱和脂肪酸（polyunsaturated fatty acids, PUFAs）。与对照组日粮配方相比，试验组日粮在育雏期与育肥期均添加了2%葡萄籽粕。试验肉鸡可自由采食与饮水。饲养试验结束后，采集血液样本用于血清生化指标测定；每组选取6只肉鸡进行屠宰，采集胸肌与腿肌样本。采用标准化方法对肌肉样本的基本化学成分、脂肪酸谱以及胆固醇含量进行检测。总多不饱和脂肪酸（PUFAs）含量显著更高（p