RNAseq analysis of mouse CD4+ regulatory Tregs in WT and ?Foxp3 mice isolated from spleen and lungs.

RNAseq analysis of mouse CD4+ regulatory Tregs in 21-day-old male WT and ?Foxp3 mice isolated from spleen and lungs. Overall design: Mouse CD4+ regulatory Tregs in 21-day-old male WT and ?Foxp3 mice were isolated from spleen and lungs by flow cytometry, as DAPI–TCRß+CD4+GFP+CD25+. 1,000 cells were double-sorted directly into lysis buffer for RNA-seq (Smart-seq2) library preparation following the standard ImmGen low-input protocol (www.immgen.org).

本数据集针对21日龄雄性野生型（Wild Type，WT）及Foxp3小鼠脾脏与肺脏中分离的CD4+调节性T细胞（CD4+ regulatory Tregs）开展RNA测序（RNA-seq）分析。实验设计：通过流式细胞术（flow cytometry），从上述两类小鼠的脾脏与肺脏中分选出表型为DAPI⁻TCRβ⁺CD4⁺GFP⁺CD25⁺的CD4+调节性T细胞；将1000个细胞经二次分选后直接加入裂解缓冲液，参照ImmGen标准低起始量实验流程（www.immgen.org）完成基于Smart-seq2技术的RNA测序文库构建。