Transcriptome analysis of RANK-positive and RANK-negative luminal progenitor subpopulations in the human breast. Homo sapiens

RANK-positive and RANK-negative luminal progenitor cells were isolated by FACS from histologically normal human breast tissue from wild-type human donors. RNA-seq gene expression profiling was used to find differentially expressed genes between the RANK-positive and RANK-negative cell populations. Overall design: Cells were isolated from 4 human patients. A paired analysis was used to compare RANK-positive and RANK-negative cells within patients.

从野生型人类供体的组织学正常人体乳腺组织中，通过荧光激活细胞分选术（Fluorescence-Activated Cell Sorting，FACS）分离得到RANK阳性与RANK阴性管腔祖细胞。采用RNA测序（RNA-seq）基因表达谱分析技术，筛选RANK阳性与RANK阴性细胞群体间的差异表达基因。实验整体设计：从4名人类患者体内分离细胞，采用配对分析方法比较每名患者体内的RANK阳性与RANK阴性细胞。