Differential Expression of CD44 Variants in Normal and AD Brain

In brief, we confirmed the specificity of commercially available CD44 antibodies by immunoblotting transfected cell samples overexpressing specific CD44 variants. We then applied the validated antibodies to examine the distribution of CD44 variant expression levels in normal and AD brain tissues using immunohistochemistry. The experimental conditions and results of this study are summarized in Table 1.

简要而言，本研究通过对过表达特定CD44变异体的转染细胞样本进行免疫印迹（immunoblotting）实验，验证了市售CD44抗体的特异性。随后，本研究使用经验证的抗体，通过免疫组织化学（immunohistochemistry）实验检测正常脑组织与阿尔茨海默病（AD）脑组织中CD44变异体的表达水平分布情况。本研究的实验条件与实验结果汇总于表1中。