Expression analysis of radio-resistant and -sensitive head and neck cancer cells

Head and Neck Squamous Cell Carcinoma (HNSCC) is a common cause of cancer death. Despite enormous technical advances in surgery and radiotherapy in the recent decade, survival of HNSCC patients has not markedly improved, with only 30% of patients diagnosed with advanced HNSCC that will survive for 5 years. This highlights the need to look into molecular processes leading to mechanisms of HNSCC radioresistance in HNSCC and identify novel radiosensitizers. To identify the lncRNAs associated with radiotherapy resistance in HNSCC, we performed RNA-seq analysis of radiation resistant and sensitive HNSCC cells. Overall design: We performed RNA-seq analysis of the isogenic SCC61 and RTR61 cell pair, as well as in two radioresistant single clones RTR61-1 and RTR61-4 derived from the total RTR61 cell population. We also used another isogenic pair, radiosensitive SCC154 cells and radioresistant RTR154 cells, and two radioresistant single clones RTR154-1 and RTR154-4 isolated from the RTR154 total population. Finally, we determined the lncRNA expression profiles of 4 radioresistant and 5 radiosensitive HNSCC cell lines

头颈部鳞状细胞癌（Head and Neck Squamous Cell Carcinoma, HNSCC）是常见的癌症致死病因之一。尽管近十年来外科与放射治疗技术取得了长足进步，但头颈鳞状细胞癌患者的生存率并未得到显著提升，确诊为晚期头颈鳞状细胞癌的患者中仅有30%可存活5年。这凸显了深入探究头颈鳞状细胞癌放射抵抗分子机制、发掘新型放射增敏剂的必要性。为鉴定与头颈鳞状细胞癌放射抵抗相关的长链非编码RNA（long non-coding RNA, lncRNA），本研究对放射抵抗型与放射敏感型头颈鳞状细胞癌细胞开展了RNA测序（RNA-seq）分析。整体实验设计：我们对同基因配对细胞系SCC61与RTR61，以及从总RTR61细胞群体中分离得到的两株放射抵抗单克隆细胞RTR61-1和RTR61-4进行了RNA测序分析；此外，本研究还使用了另一组同基因配对细胞系：放射敏感型SCC154细胞与放射抵抗型RTR154细胞，以及从RTR154总细胞群体中分离得到的两株放射抵抗单克隆细胞RTR154-1和RTR154-4；最终，本研究明确了4株放射抵抗型与5株放射敏感型头颈鳞状细胞癌细胞系的长链非编码RNA表达谱。