mRNA data - Unique miRNome and Transcriptome Profiles Underlie Microvascular Heterogeneity in Mouse Kidney

Endothelial cells in the blood vessels in the kidney exert different functions depending on the (micro)vascular bed they are located in. These functional differences are likely a result of differential microRNA and mRNA transcription patterns, yet the identity of these molecules is not well known. We zoomed in on the endothelial cells of microvascular compartments in mouse renal cortex by laser microdissecting the microvessels prior to (small) RNA sequencing analyses. By these means, we characterised microRNA and mRNA transcription profiles of arterioles, glomeruli, peritubular capillaries, and post-capillary venules. RT-qPCR, in situ hybridisation, and immunohistochemistry were used to validate sequencing results. Unique microRNA and mRNA transcription profiles were found in all microvascular compartments, with dedicated marker microRNAs and mRNAs showing enriched transcription in a single microvascular compartment. In situ hybridisation validated localisation of microRNAs mmu-miR-140-3p in arterioles, of mmu-miR-322-3p in glomeruli, and of mmu-miR-451a in post-capillary venules. Immunohistochemical staining showed that von Willebrand Factor protein was mainly expressed in arterioles and post-capillary venules, while GABRB1 expression was enriched in glomeruli and IGF1 in post-capillary venules. Our study shows that microvascular endothelial heterogeneity in mouse kidney is a result of a combination of differentially expressed microRNAs and mRNAs. The identified profiles provide important molecular information to take into account for future studies into microvascular engagement in health and disease. Gene expression analysis in microvascular compartments from healthy mouse kidney cortex (arterioles, glomeruli, peritubular capillaries, post-capillary venules) to reveal microvascular-compartment specific gene expression profiles

肾脏血管内皮细胞根据其所处的（微）血管床位置，发挥各异的功能。这类功能差异大概率源于差异表达的微小RNA（microRNA）和信使RNA（mRNA）转录模式，但目前此类功能性分子的具体种类仍未得到充分阐明。我们通过激光显微切割技术分离小鼠肾皮质微血管室的内皮细胞，随后开展（小）RNA测序分析。借此，我们对小动脉、肾小球、肾小管周围毛细血管以及毛细血管后静脉的微小RNA和信使RNA转录谱进行了系统表征。采用实时定量聚合酶链反应（RT-qPCR）、原位杂交（in situ hybridisation）与免疫组织化学（immunohistochemistry）技术对测序结果进行验证。研究发现，所有微血管室均拥有独特的微小RNA和信使RNA转录谱，专属的标记性微小RNA与信使RNA仅在单一微血管室中呈现富集表达。原位杂交实验验证了mmu-miR-140-3p定位于小动脉、mmu-miR-322-3p定位于肾小球，以及mmu-miR-451a定位于毛细血管后静脉。免疫组织化学染色结果显示，血管性血友病因子（von Willebrand Factor）蛋白主要在小动脉与毛细血管后静脉中表达，而GABRB1（γ-氨基丁酸A型受体亚基β1）在肾小球中富集，胰岛素样生长因子1（IGF1）则在毛细血管后静脉中高表达。本研究证实，小鼠肾脏微血管内皮细胞的异质性是差异表达的微小RNA与信使RNA共同作用的结果。本次鉴定得到的转录谱可为未来研究微血管在健康与疾病状态中的作用提供关键的分子信息。本数据集源自健康小鼠肾皮质微血管室（小动脉、肾小球、肾小管周围毛细血管、毛细血管后静脉）的基因表达分析，旨在揭示微血管室特异性的基因表达谱。