resting and IL2 activated human natural killer cells. Homo sapiens

The aim of the study was to investigate the activation of human NK cells by IL2 through analyzing the global gene expression at different time points (0, 2, 8 and 24 hours) after culture with the cytokine IL2 at 100 IU/ml. NK cells with the CD56+/CD16+ and CD3- phenotype were negatively selected by immunomagnetic beads and re-examined by flow-cytometry to ensure greater than 90% purity . Keywords: resting and IL2 activated NK cells(time series) Overall design: RNA was extracted and pooled from 4 different donors, amplified and labled according to the manufacturer’s instruction (GeneChipU133plus2® , Affymetrix Inc, CA).

本研究旨在通过分析人类自然杀伤细胞（natural killer cells, NK）经100 IU/ml浓度的白细胞介素2（interleukin-2, IL2）细胞因子培养后不同时间点（0、2、8及24小时）的全基因表达谱，探究IL2对NK细胞的激活作用。
本研究通过免疫磁珠阴性分选获得CD56+/CD16+且CD3-表型的NK细胞，并经流式细胞术复检以确保细胞纯度不低于90%。
关键词：静息状态及IL2激活的NK细胞（时间序列）
实验整体设计：从4名不同供体中提取总RNA并混合，按照美国加利福尼亚州Affymetrix公司GeneChipU133plus2®芯片的操作指南进行RNA扩增与标记。