Altered histone acetylation patterns in pancreatic cancer cell lines induce subtype-specific transcriptomic and phenotypic changes.

Background: Pancreatic ductal adenocarcinoma (PDAC) is often diagnosed at advanced tumor stages with chemotherapy as the only treatment option. Large-scale gene expression studies have defined two major PDAC subtypes, a classical and basal-like, which differ in their response to chemotherapy. The transcriptional networks, which define the molecular PDAC subtypes, are regulated by epigenetic modifications. Given the reversible nature of the epigenome, we aim to determine if drug-induced epigenetic reprogramming of pancreatic cancer cells affects PDAC subtype identity and chemosensitivity. Overall design: Steady-state RNA measurement in varous PDAC cell lines with and without treatment

背景：胰腺导管腺癌（Pancreatic ductal adenocarcinoma, PDAC）通常在肿瘤晚期阶段才被确诊，且化疗是唯一的治疗选择。大规模基因表达谱研究已明确两种主要的PDAC亚型：经典型与基底样型，二者对化疗的响应存在显著差异。定义胰腺导管腺癌分子亚型的转录网络，受表观遗传修饰的调控。鉴于表观基因组具有可逆性，本研究旨在探究药物诱导的胰腺癌细胞表观遗传重编程，是否会影响PDAC的亚型特征与化疗敏感性。实验设计：在经/未经药物处理的多种PDAC细胞系中，检测其稳态RNA水平。